Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase.

@article{Kanegae1995EfficientGA,
  title={Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase.},
  author={Yumi Kanegae and G Lee and Yuko Sato and Mika Tanaka and Masamichi Nakai and Toshiyuki Sakaki and Sumio Sugano and Izumu Saito},
  journal={Nucleic acids research},
  year={1995},
  volume={23 19},
  pages={3816-21}
}
A recombinant adenovirus (Ad) expressing Cre recombinase derived from bacteriophage P1 was constructed. To assay the Cre activity in mammalian cells, another recombinant Ad bearing an on/off-switching reporter unit, where a LacZ-expression unit can be activated by the Cre-mediated excisional deletion of an interposed stuffer DNA, was also constructed. Co-infection experiments together with the Cre-expressing and the reporter recombinant Ads showed that the Cre-mediated switching of gene… CONTINUE READING
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