Efficient extension of slipped DNA intermediates by DinB is required to escape primer template realignment by DnaQ.

Abstract

We show that Escherichia coli DinB polymerase, which creates single-base deletions, prefers to extend slipped DNA substrates with the skipped base at the -4 position. A DinB(Y79L) variant, which extends these substrates less efficiently in vitro, allows the proofreading function of polymerase III to reverse their formation in vivo. 
DOI: 10.1128/JB.00005-11

Cite this paper

@article{Foti2011EfficientEO, title={Efficient extension of slipped DNA intermediates by DinB is required to escape primer template realignment by DnaQ.}, author={James J. Foti and Graham C Walker}, journal={Journal of bacteriology}, year={2011}, volume={193 10}, pages={2637-41} }