Efficient expression system of human recombinant laminin-5.

@article{Kariya2002EfficientES,
  title={Efficient expression system of human recombinant laminin-5.},
  author={Yoshinobu Kariya and Kumiko Ishida and Yoshiaki Tsubota and Yukiko Nakashima and Tomomi Hirosaki and Takashi Ogawa and Kaoru Miyazaki},
  journal={Journal of biochemistry},
  year={2002},
  volume={132 4},
  pages={607-12}
}
Laminin-5, a heterotrimer of laminin alpha3, beta3, and gamma2 chains, is an essential component of various epithelial basement membranes, and it strongly promotes cellular adhesion and motility in vitro. In this study, we established an efficient expression system of human recombinant laminin-5 (rLN5), in which full-length cDNAs encoding the human laminin alpha3, beta3, and gamma2 chains were introduced into the human embryonic kidney cell line HEK293. rLN5 was purified from the conditioned… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 20 extracted citations

Similar Papers

Loading similar papers…