Efficient expression screening of human membrane proteins in transiently transfected Human Embryonic Kidney 293S cells.

@article{Chaudhary2011EfficientES,
  title={Efficient expression screening of human membrane proteins in transiently transfected Human Embryonic Kidney 293S cells.},
  author={Sarika Chaudhary and John Pak and Bj\orn Panyella Pedersen and Lois J Bang and Liye Zhang and Samantha M M Ngaw and Raissa G Green and Vinay Sharma and Robert Michael Stroud},
  journal={Methods},
  year={2011},
  volume={55 4},
  pages={273-80}
}
It is often an immense challenge to overexpress human membrane proteins at levels sufficient for structural studies. The use of Human Embryonic Kidney 293 (HEK 293) cells to express full-length human membrane proteins is becoming increasingly common, since these cells provide a near-native protein folding and lipid environment. Nevertheless, the labor intensiveness and low yields of HEK 293 cells and other mammalian cell expression systems necessitate the screening for suitable expression as… CONTINUE READING

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