Efficient expression of tum− antigen P91A by transfected subgenic fragments

Mutagen treatment of mouse P815 tumor cells produces immunogenic mutants that express new transplantation antigens (tum− antigens) recognized by cytolytic T cells. The gene encoding tum− antigen P91A comprises 12 exons and a mutation located in exon 4 is responsible for the production of a new antigenic peptide. Transfection experiments showed that the… CONTINUE READING