Efficient expression of human beta-interferon in Namalwa KJM-1 cells adapted to serum-free medium by a dhfr gene coamplification method

Abstract

We previously reported the expression of human beta-interferon (β-IFN) (Miyajiet al., 1989) and human lymphotoxin (Miyajiet al., 1990) in Namalwa KJM-1 cells adapted to serum-free medium. To establish an efficient gene expression system, a dihydrofolate reductase (dhfr) gene coamplification method was applied to this cell line. A β-IFN expression plasmid… (More)
DOI: 10.1007/BF00365098