Efficient establishment of human embryonic stem cell lines and long-term maintenance with stable karyotype by enzymatic bulk passage.

@article{Suemori2006EfficientEO,
  title={Efficient establishment of human embryonic stem cell lines and long-term maintenance with stable karyotype by enzymatic bulk passage.},
  author={Hirofumi Suemori and Kentaro Yasuchika and Kouichi Hasegawa and Tsuyoshi Fujioka and Norihiro Tsuneyoshi and Norio Nakatsuji},
  journal={Biochemical and biophysical research communications},
  year={2006},
  volume={345 3},
  pages={926-32}
}
Human ES (hES) cell lines are considered to be a valuable resource for medical research and for applications in cell therapy and drug discovery. For such utilization of hES cells to be realized, however, protocols involved in the use of hES cells, such as those for establishment, propagation, and cryopreservation, have still to be improved. Here, we report on an efficient method for the establishment of hES cell lines and its detailed characterization. Additionally, we developed a new bulk… CONTINUE READING
Highly Influential
This paper has highly influenced 19 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS