Efficient enzymatic synthesis of LNA-modified DNA duplexes using KOD DNA polymerase.

@article{Veedu2009EfficientES,
  title={Efficient enzymatic synthesis of LNA-modified DNA duplexes using KOD DNA polymerase.},
  author={Rakesh N Veedu and Birte Vester and Jesper Wengel},
  journal={Organic & biomolecular chemistry},
  year={2009},
  volume={7 7},
  pages={1404-9}
}
Three different LNA-nucleoside triphosphates, LNA-TTP, LNA-ATP and LNA-5-methyl-CTP, were investigated as substrates for KOD DNA polymerase. The results reveal that KOD DNA polymerase is an efficient catalyst for template directed synthesis of DNA oligonucleotide duplexes containing a large number of LNA nucleotides by primer extension reactions. Furthermore, KOD DNA polymerase is shown to be suitable for the PCR amplification of an LNA-modified DNA duplex.