Efficient ends-out gene targeting in Drosophila.


In this report, we describe several approaches to improve the scalability and throughput of major genetic crosses in ends-out gene targeting. We generated new sets of targeting vectors and fly stocks and introduced a novel negative selection marker that drastically reduced the frequency of false-positive targeting candidates. 
DOI: 10.1534/genetics.108.090563

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@article{Huang2008EfficientEG, title={Efficient ends-out gene targeting in Drosophila.}, author={Juan Huang and Wenke Zhou and Annie M M Watson and Y S Jan and Yang Hong}, journal={Genetics}, year={2008}, volume={180 1}, pages={703-7} }