Efficient encapsulation of antisense oligonucleotides in lipid vesicles using ionizable aminolipids: formation of novel small multilamellar vesicle structures.

@article{Semple2001EfficientEO,
  title={Efficient encapsulation of antisense oligonucleotides in lipid vesicles using ionizable aminolipids: formation of novel small multilamellar vesicle structures.},
  author={Sean C Semple and Sandy K Klimuk and Troy O Harasym and Nancy P Dos Santos and Steven M Ansell and Kim F. Wong and Norbert Maurer and Heiko Stark and Pieter R Cullis and Michael J. Hope and Peter Scherrer},
  journal={Biochimica et biophysica acta},
  year={2001},
  volume={1510 1-2},
  pages={152-66}
}
Typical methods used for encapsulating antisense oligodeoxynucleotides (ODN) and plasmid DNA in lipid vesicles result in very low encapsulation efficiencies or employ cationic lipids that exhibit unfavorable pharmacokinetic and toxicity characteristics when administered intravenously. In this study, we describe and characterize a novel formulation process that utilizes an ionizable aminolipid (1,2-dioleoyl-3-dimethylammonium propane, DODAP) and an ethanol-containing buffer system for… CONTINUE READING
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