Efficient detection of DNA polymorphism in cabbage and rice cultivars by PCR-RF-SSCP (PRS)

@article{Sato2002EfficientDO,
  title={Efficient detection of DNA polymorphism in cabbage and rice cultivars by PCR-RF-SSCP (PRS)},
  author={Yo-ichiro Sato and Takeshi Nishio},
  journal={Plant Cell Reports},
  year={2002},
  volume={21},
  pages={276-281}
}
PCR (polymerase chain reaction)-RF(restriction fragment)-SSCP (single-strand conformation polymorphism) – designated here as PRS – is a combined method of SSCP and PCR-RFLP (restriction fragment length polymorphism) – designated as CAPS (cleaved amplified polymorphic sequence) – and was efficient in detecting intraspecific variation of the SLR1 gene in Brassica oleracea. One to six nucleotide changes in restriction fragments of the SLR1 gene were detected as different bands in PRS. In an… CONTINUE READING
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