Efficient de novo assembly of highly heterozygous genomes from whole-genome shotgun short reads.

@article{Kajitani2014EfficientDN,
  title={Efficient de novo assembly of highly heterozygous genomes from whole-genome shotgun short reads.},
  author={Rei Kajitani and Kouta Toshimoto and Hideki Noguchi and Atsushi Toyoda and Yoshitoshi Ogura and Miki Okuno and Mitsuru Yabana and Masayuki Harada and Eiji Nagayasu and Haruhiko Maruyama and Yuji Kohara and Asao Fujiyama and Tetsuya Hayashi and Takehiko Itoh},
  journal={Genome research},
  year={2014},
  volume={24 8},
  pages={
          1384-95
        }
}
Although many de novo genome assembly projects have recently been conducted using high-throughput sequencers, assembling highly heterozygous diploid genomes is a substantial challenge due to the increased complexity of the de Bruijn graph structure predominantly used. To address the increasing demand for sequencing of nonmodel and/or wild-type samples, in most cases inbred lines or fosmid-based hierarchical sequencing methods are used to overcome such problems. However, these methods are costly… CONTINUE READING
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