Efficient colonization of plant roots by the plant growth promoting bacterium Bacillus amyloliquefaciens FZB42, engineered to express green fluorescent protein.

@article{Fan2011EfficientCO,
  title={Efficient colonization of plant roots by the plant growth promoting bacterium Bacillus amyloliquefaciens FZB42, engineered to express green fluorescent protein.},
  author={Ben Fan and Xiao Hua Chen and Anto Budiharjo and Wilfrid Bleiss and Joachim Vater and Rainer Borriss},
  journal={Journal of biotechnology},
  year={2011},
  volume={151 4},
  pages={303-11}
}
A single copy of the gfp gene linked with the P(spac) promoter and flanked by the terminal FZB42 amyE sequences was stably integrated into the chromosome of plant growth promoting bacterium Bacillus amyloliquefaciens FZB42 via homologous recombination. A spontaneous mutant, FB01mut, emitting bright fluorescence was detected among the transformants and found suitable for colonization experiments performed with Zea mays, Arabidopsis thaliana and Lemna minor. Real-time RT-PCR revealed that FB01mut… CONTINUE READING
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