Efficient biallelic mutation in porcine parthenotes using a CRISPR-Cas9 system.

@article{Tao2016EfficientBM,
  title={Efficient biallelic mutation in porcine parthenotes using a CRISPR-Cas9 system.},
  author={Li Tao and Mingyao Yang and Xiaodong Wang and Zhenni Zhang and Zhonghong Wu and Jian-hui Tian and Lei An and Shumin Wang},
  journal={Biochemical and biophysical research communications},
  year={2016},
  volume={476 4},
  pages={225-229}
}
The parthenotes represent ideal models mimicking the embryonic development and characterizing the function of maternal genomes as well as an alternative source of pluripotent cell lines. Besides, parthenogenetically activated (PA) embryos serve as a rapid assay system to maximize the efficiency of generating genetically modified pig CRISPR/Cas9 system, an… CONTINUE READING