Efficient and rapid protein expression and purification of small high disulfide containing sweet protein brazzein in E. coli.

@article{AssadiPorter2008EfficientAR,
  title={Efficient and rapid protein expression and purification of small high disulfide containing sweet protein brazzein in E. coli.},
  author={Fariba M Assadi-Porter and Sammy Patry and John L. Markley},
  journal={Protein expression and purification},
  year={2008},
  volume={58 2},
  pages={263-8}
}
Brazzein protein comes from an edible fruit, which has a long history of being a staple in the local human diet in Africa. The attractive features of brazzein as a potential commercial sweetener include its small size (53 amino acid residues), its stability over wide ranges of temperature and pH, and the similarity of its sweetness to sucrose. Heterologous production of brazzein is complicated by the fact that the protein contains four disulfide bridges and requires a specific N-terminal… CONTINUE READING