Efficient and genotype-independent Agrobacterium--mediated tomato transformation.

  title={Efficient and genotype-independent Agrobacterium--mediated tomato transformation.},
  author={Sung Hun Park and Jay L. Morris and Jung Eun Park and Kendal D Hirschi and Roberta H. Smith},
  journal={Journal of plant physiology},
  volume={160 10},
An efficient method to transform five cultivars of tomato (Lycopersicon esculentum), Micro-Tom, Red Cherry, Rubion, Piedmont, and E6203 is reported. A comparison was made of leaf, cotyledon, and hypocotyl explants on 7 different regeneration media without Agrobacterium tumefaciens cocultivation and on 11 different media with cocultivation. Although all cultivars and explants formed callus and regenerated on the initial 7 media, cocultivation with A. tumefaciens significantly reduced the callus… CONTINUE READING
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