Efficient and Rapid Affinity Purification of Proteins Using Recombinant Fusion Proteases


In the affinity purification of recombinant fusion proteins, the rate-limiting step is usually the efficient proteolytic cleavage and removal of the affinity tail and the protease from the purified recombinant protein. We have developed a rapid, convenient and efficient method of affinity purification which can overcome this limitation. In one example of… (More)
DOI: 10.1038/nbt0694-601