Efficient adventitious shoot regeneration and somatic embryogenesis in pea

@article{zcan2004EfficientAS,
  title={Efficient adventitious shoot regeneration and somatic embryogenesis in pea},
  author={Sebahattin {\"O}zcan and Mehdi Barghchi and S. Firek and John Draper},
  journal={Plant Cell, Tissue and Organ Culture},
  year={2004},
  volume={34},
  pages={271-277}
}
A rapid and simple method for adventitious shoot regeneration and somatic embryogenesis from immature cotyledon explants of pea (Pisum sativum L.) is described. Cotyledon size and the explant orientation to the medium surface were shown to have a clear effect on shoot regeneration. The highest frequency of shoot regeneration was achieved when the distal end of the greenest cotyledons (7–8 mm in size) were placed in contact with the agar surface. Shoots rooted at a frequency of 80–90% and grew… CONTINUE READING
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