Efficient adhesion-based plasma membrane isolation for cell surface N-glycan analysis.

@article{Mun2013EfficientAP,
  title={Efficient adhesion-based plasma membrane isolation for cell surface N-glycan analysis.},
  author={Ji-Young Mun and Kyung Jin Lee and Hoon Seo and Min-Sun Sung and Yee Sook Cho and Seung Goo Lee and Ohsuk Kwon and Doo-Byoung Oh},
  journal={Analytical chemistry},
  year={2013},
  volume={85 15},
  pages={7462-70}
}
Glycans, which decorate cell surfaces, play crucial roles in various physiological events involving cell surface recognition. Despite the importance of surface glycans, most analyses have been performed using total cells or whole membranes rather than plasma membranes due to difficulties related to isolation. In the present study, we employed an adhesion-based method for plasma membrane isolation to analyze N-glycans on cell surfaces. Cells were attached to polylysine-coated glass plates and… CONTINUE READING
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