Efficient activation of T cells by human monocyte-derived dendritic cells (HMDCs) pulsed with Coxiella burnetii outer membrane protein Com1 but not by HspB-pulsed HMDCs

@article{Wang2011EfficientAO,
  title={Efficient activation of T cells by human monocyte-derived dendritic cells (HMDCs) pulsed with Coxiella burnetii outer membrane protein Com1 but not by HspB-pulsed HMDCs},
  author={Ying Wang and Xiaolu Xiong and Deping Wu and Xile Wang and Bohai Wen},
  journal={BMC Immunology},
  year={2011},
  volume={12},
  pages={52 - 52}
}
BackgroundCoxiella burnetii is an obligate intracellular bacterium and the etiologic agent of Q fever; both coxiella outer membrane protein 1 (Com1) and heat shock protein B (HspB) are its major immunodominant antigens. It is not clear whether Com1 and HspB have the ability to mount immune responses against C. burnetii infection.ResultsThe recombinant proteins Com1 and HspB were applied to pulse human monocyte-derived dendritic cells (HMDCs), and the pulsed HMDCs were used to stimulate isogenic… 
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Mice immunized with bone marrow-derived dendritic cells stimulated with recombinant Coxiella burnetii Com1 and Mip demonstrate enhanced bacterial clearance in association with a Th1 immune response.
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Humoral and cellular immune responses to C. burnetii infection in pregnant goats are demonstrated and this humoral immune response may be useful in the early detection of C. burningetii-infected pregnant goats.
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The Effect of C. burnetii Infection on the Cytokine Response of PBMCs from Pregnant Goats
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It is shown that goats initiate a robust pro-inflammatory immune response against C. burnetii in vitro, and PBMCs from C.Burnetii infected goats show augmented pro- inflammatory cytokine responses compared to PBMCS from non-infected goats, however, despite this pro- inflammation, goats are not capable of clearing the C.burnetii infection.
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Evidence indicates that infected DCs are not sufficiently activated, do not undergo maturation and do not produce expected proinflammatory cytokines, which indicates that pathogenic intracellular bacteria can subvert DC function and gain the advantage of an ineffective host immune reaction.
Evaluation of the Diagnostic Potential of Recombinant Coxiella burnetii Com1 in an ELISA for the Diagnosis of Q Fever in Sheep, Goats and Cattle
TLDR
Re recombinant Com1 can provide a basis for more sensitive and specific diagnostic tools in veterinary medicine, and correspond to excellent, outstanding and acceptable discrimination of positive and negative sera.
Coxiella burnetii in pregnant goats
TLDR
Investigation of the epidemiology and pathogenesis of C. burnetii infection in goats in the recent large outbreak of Q fever in the Netherlands confirms dairy goats and sheep as the source of the Dutch Q fever outbreak, and suggests species-specific genotypes for roe deer and cattle that are unrelated to the Dutch outbreak genotype.
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References

SHOWING 1-10 OF 55 REFERENCES
Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor alpha
TLDR
Cultured DCs are as efficient as antigen-specific B cells in presenting tetanus toxoid (TT) to specific T cell clones and their efficiency of antigen presentation can be further enhanced by specific antibodies via FcR- mediated antigen uptake.
Coxiella burnetii antigen-stimulated dendritic cells mediated protection against Coxiella burnetii in BALB/c mice.
TLDR
It is suggested that PIIAg is far superior to PIAg in activating BMDCs to confer protection against C. burnetii in vivo, whereas Com1 and SecB are protective antigens because Com1- or SecB-pulsed BM DCs confer partial protection.
Antigen dose, type of antigen‐presenting cell and time of differentiation contribute to the T helper 1/T helper 2 polarization of naive T cells
TLDR
Evidence is provided to show that antigen dose, type of antigen‐presenting cell and time of differentiation can contribute to T‐cell differentiation.
Mycobacterium tuberculosis heat‐shock protein 70 impairs maturation of dendritic cells from bone marrow precursors, induces interleukin‐10 production and inhibits T‐cell proliferation in vitro
TLDR
The hypothesis that TBhsp70 does not have inflammatory potential, but rather has immunosuppressive properties is supported, as demonstrated by the demonstrated inhibition of phytohaemagglutinin‐induced T‐cell proliferation.
Preferential Costimulation by CD80 Results in IL-10-Dependent TGF-β1+-Adaptive Regulatory T Cell Generation1
TLDR
This study demonstrates that not only did blockade of CD86 upon Ag presentation by bone marrow-derived dendritic cells (DC) to OVA-specific T cells result in induction of hyporesponsive T cells but also that these T cells could suppress the proliferative response of effector T cells.
Elevated levels of Legionella pneumophila stress protein Hsp60 early in infection of human monocytes and L929 cells correlate with virulence
TLDR
V virulent, but not NaCl-tolerant avirulent, strains of L. pneumophila respond to host-cell-associated environmental signals early in the course of infection, which includes increased synthesis and possibly extracellular secretion of Hsp60 concomitant with repression of the expression of other genes, including ompS.
T Cells Are Essential for Bacterial Clearance, and Gamma Interferon, Tumor Necrosis Factor Alpha, and B Cells Are Crucial for Disease Development in Coxiella burnetii Infection in Mice
TLDR
The results suggest that T cells are critical for clearance of C. burnetii, either NM I or NM II, that IFN-γ and TNF-α are essential for the early control of infection, and that B cells are important for the prevention of tissue damage.
Interleukin-10 Stimulates Coxiella burnetiiReplication in Human Monocytes through Tumor Necrosis Factor Down-Modulation: Role in Microbicidal Defect of Q Fever
TLDR
It is suggested that interleukin-10 enables monocytes to support C. burnetii replication and to favor the development of chronic Q fever.
Coxiella burnetii Survival in THP-1 Monocytes Involves the Impairment of Phagosome Maturation: IFN-γ Mediates its Restoration and Bacterial Killing1
TLDR
It is shown that C. burnetii survival in THP-1 monocytes was not related to phagosomal pH because bacterial vacuoles were acidic independently of C. burningetii virulence, and that IFN-γ mediates C.Burnetii killing via two distinct mechanisms, phagosome maturation, andphagosome alkalinization.
Lipopolysaccharide from Coxiella burnetii Is Involved in Bacterial Phagocytosis, Filamentous Actin Reorganization, and Inflammatory Responses through Toll-Like Receptor 41
TLDR
Results demonstrate that TLR4 controls early events of C. burnetii infection such as macrophage phagocytosis, granuloma formation, and cytokine production.
...
1
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3
4
5
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