Efficient UV stimulation of yeast integrative transformation requires damage on both plasmid strands

@article{Ninkovi1994EfficientUS,
  title={Efficient UV stimulation of yeast integrative transformation requires damage on both plasmid strands},
  author={Milena Ninkovi{\'c} and M. Ala{\vc}evi{\'c} and Francis Fabre and Zoran Zgaga},
  journal={Molecular and General Genetics MGG},
  year={1994},
  volume={243},
  pages={308-314}
}
The nature of UV-induced pre-recombinational structures was studied using transformation of Saccharomyces cerevisiae cells with non-replicative plasmids. Transformation by double-stranded plasmids irradiated with UV was stimulated up to 50-fold, and both plasmid integration and conversion of the mutated chromosomal selective gene were found to be equally increased. The stimulation observed with such ‘totally’ irradiated plasmids was not found with plasmids bearing lesions in only one strand… CONTINUE READING

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