Efficient Transformation of the Cephamycin C Producer Nocardia lactamdurans and Development of Shuttle and Promoter-Probe Cloning Vectors.

@article{Kumar1994EfficientTO,
  title={Efficient Transformation of the Cephamycin C Producer Nocardia lactamdurans and Development of Shuttle and Promoter-Probe Cloning Vectors.},
  author={C V Kumar and Juan Jos{\'e} R. Coque and Juan F. Martin},
  journal={Applied and environmental microbiology},
  year={1994},
  volume={60 11},
  pages={4086-93}
}
A high transformation efficiency (1 x 10 to 7 x 10 transformants per mug of DNA) of Nocardia lactamdurans LC411 was obtained by direct treatment of mycelium with polyethylene glycol 1000 and cesium chloride. A variety of vectors from Streptomyces lividans, Brevibacterium lactofermentum, Rhodococcus fascians, and a Nocardia (Amycolatopsis) sp. were tested; transformants could be obtained only with vectors derived from an endogenous plasmid of the Amycolatopsis sp. strain DSM 43387. Vectors… CONTINUE READING