Efficient Screening and Optimization of Membrane Protein Production in Escherichia coli.

Abstract

Escherichia coli is one of the most widely used expression hosts for membrane proteins. However, establishing conditions for its recombinant production of membrane proteins remains difficult. Attempts to produce membrane proteins frequently result in either no expression or expression as misfolded aggregates. We developed an efficient pipeline for improving… (More)
DOI: 10.1016/bs.mie.2017.05.011

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