Efficient Reassignment of a Frequent Serine Codon in Wild-Type Escherichia coli.

@article{Ho2016EfficientRO,
  title={Efficient Reassignment of a Frequent Serine Codon in Wild-Type Escherichia coli.},
  author={Joanne M Ho and Noah M. Reynolds and Keith D. Rivera and Morgan P. Connolly and Li-Tao Guo and Jiqiang Ling and Darryl J. Pappin and George M Church and Dieter S{\"o}ll},
  journal={ACS synthetic biology},
  year={2016},
  volume={5 2},
  pages={163-71}
}
Expansion of the genetic code through engineering the translation machinery has greatly increased the chemical repertoire of the proteome. This has been accomplished mainly by read-through of UAG or UGA stop codons by the noncanonical aminoacyl-tRNA of choice. While stop codon read-through involves competition with the translation release factors, sense codon reassignment entails competition with a large pool of endogenous tRNAs. We used an engineered pyrrolysyl-tRNA synthetase to incorporate 3… CONTINUE READING
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