Efficient Production of 2-Oxobutyrate from 2-Hydroxybutyrate by Using Whole Cells of Pseudomonas stutzeri Strain SDM

@article{Gao2010EfficientPO,
  title={Efficient Production of 2-Oxobutyrate from 2-Hydroxybutyrate by Using Whole Cells of Pseudomonas stutzeri Strain SDM},
  author={Chao Gao and Wen Zhang and Chuanjuan Lv and Lixiang Li and Cuiqing Ma and Chunhui Hu and Ping Xu},
  journal={Applied and Environmental Microbiology},
  year={2010},
  volume={76},
  pages={1679 - 1682}
}
  • Chao Gao, Wen Zhang, P. Xu
  • Published 15 January 2010
  • Biology, Engineering
  • Applied and Environmental Microbiology
ABSTRACT 2-Oxobutyrate is an important intermediate in the chemical, drug, and food industries. Whole cells of Pseudomonas stutzeri SDM, containing NAD-independent lactate dehydrogenases, effectively converted 2-hydroxybutyrate into 2-oxobutyrate. Under optimal conditions, the biocatalytic process produced 2-oxobutyrate at a high concentration (44.4 g liter−1) and a high yield (91.5%). 
Highly efficient production of 2-ketobutyrate from 2-hydroxybutyrate by resting cells of Pseudomonas stutzeri DEH130
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TLDR
A 2.94-Mb assembly of Gluconobacter oxydans genome sequence is presented, which might provide further insights into the molecular mechanism of its biocatalysis in order to further improve its biotechnological applications.
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TLDR
Since the side reaction catalyzed by the NAD-independent l-lactate dehydrogenase was eliminated in whole cells of recombinant P. putida KT2440, two important l-2-hydroxy-carboxylates were produced in high yield and high optical purity by kinetic resolution of racemic 2-Hydroxy carboxylic acids.
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