Efficient PCR-based gene disruption in Saccharomyces strains using intergenic primers.

@article{Reid2002EfficientPG,
  title={Efficient PCR-based gene disruption in Saccharomyces strains using intergenic primers.},
  author={Robert J. D. Reid and Ivana Sunjevaric and Mehdi A Keddache and Rodney Rothstein},
  journal={Yeast},
  year={2002},
  volume={19 4},
  pages={319-28}
}
Gene disruptions are a vital tool for understanding Saccharomyces cerevisiae gene function. An arrayed library of gene disruption strains has been produced by a consortium of yeast laboratories; however their use is limited to a single genetic background. Since the yeast research community works with several different strain backgrounds, disruption libraries in other common laboratory strains are desirable. We have developed simple PCR-based methods that allow transfer of gene disruptions from… CONTINUE READING