Efficient Method of Agrobacterium-mediated Transformation for Triticale (x Triticosecale Wittmack)

@article{NadolskaOrczyk2004EfficientMO,
  title={Efficient Method of Agrobacterium-mediated Transformation for Triticale (x Triticosecale Wittmack)},
  author={Anna Nadolska-Orczyk and Anna Przetakiewicz and Kristin N. Kopera and Agnieszka Bińka and Wacław Orczyk},
  journal={Journal of Plant Growth Regulation},
  year={2004},
  volume={24},
  pages={2-10}
}
Transgenic plants of triticale cv. Wanad were obtained after transformation using three combinations of strain/vectors. Two of them were hypervirulent Agrobacterium tumefaciens strains (AGL1 and EHA101) with vectors containing bar under maize ubiquitin 1 promoter (pDM805), and both hpt under p35S and nptII under pnos (pGAH). The third one was a regular LBA4404 strain containing super-binary plasmid pTOK233 with selection genes the same as in pGAH. The efficiency of transformation was from 0 to… 

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