Efficient Generation of Gene-Modified Pigs Harboring Precise Orthologous Human Mutation via CRISPR/Cas9-Induced Homology-Directed Repair in Zygotes.

@article{Zhou2016EfficientGO,
  title={Efficient Generation of Gene-Modified Pigs Harboring Precise Orthologous Human Mutation via CRISPR/Cas9-Induced Homology-Directed Repair in Zygotes.},
  author={Xiaoyang Zhou and Lulu Wang and Yinan Du and Fei Xie and Liang Li and Yu Sun John Liu and Chuanhong Liu and Shiqiang Wang and Shibing Zhang and Xingxu Huang and Yong Wang and Hong Wei},
  journal={Human mutation},
  year={2016},
  volume={37 1},
  pages={110-8}
}
Precise genetic mutation of model animals is highly valuable for functional investigation of human mutations. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9)-induced homology-directed repair (HDR) is usually used for precise genetic mutation, being limited by the relatively low efficiency compared with that of non-homologous end joining (NHEJ). Although inhibition of NHEJ was shown to enhance HDR-derived mutation, in this work, without inhibition of… CONTINUE READING
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