Efficient GFP mutations profoundly affect mRNA transcription and translation rates.

@article{Sacchetti2001EfficientGM,
  title={Efficient GFP mutations profoundly affect mRNA transcription and translation rates.},
  author={Andrea Sacchetti and T El Sewedy and Ashraf F Nasr and Saverio Alberti},
  journal={FEBS letters},
  year={2001},
  volume={492 1-2},
  pages={151-5}
}
Green fluorescent protein (GFP) variants with higher expression efficiencies have been generated by mutagenesis. Favorable mutations often improve the folding of GFP. However, an effect on protein folding fails to explain the efficiency of several other GFP mutations. In this work, we demonstrate that mutations of the GFP open reading frame and untranslated regions profoundly affect mRNA transcription and translation efficiencies. The removal of the GFP 5' untranslated region halves the… CONTINUE READING

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