Efficient Depletion of Essential Gene Products for Loss-of-Function Studies in Embryonic Stem Cells.

Abstract

The development of the CRISPR/Cas9 technology has provided powerful methods to target genetic alterations. However, investigating the function of genes essential for cell survival remains problematic, because genetic ablation of these genes results in cell death. As a consequence, cells recombined at the targeted gene and fully depleted of the gene product… (More)
DOI: 10.1007/978-1-4939-7108-4_7

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