Efficient DNA extraction for HPV genotyping in formalin-fixed, paraffin-embedded tissues.

Abstract

DNA from archived FFPE can be used for papillomavirus genotyping, but potential problems include paraffin as a physical barrier, DNA cross-linking, and PCR inhibitors. To address these complications, we combined a commercially available DNA isolation kit (Qiagen DNeasy) with a heat treatment and evaluated the resulting DNA with regards to HPV typing. DNA… (More)
DOI: 10.1016/j.jmoldx.2011.03.007

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