Efficient Cre-mediated deletion in cardiac progenitor cells conferred by a 3'UTR-ires-Cre allele of the homeobox gene Nkx2-5.

@article{Stanley2002EfficientCD,
  title={Efficient Cre-mediated deletion in cardiac progenitor cells conferred by a 3'UTR-ires-Cre allele of the homeobox gene Nkx2-5.},
  author={Edouard G. Stanley and Christine Biben and Andrew G. Elefanty and Louise Barnett and Frank Koentgen and Lorraine Robb and Richard P. Harvey},
  journal={The International journal of developmental biology},
  year={2002},
  volume={46 4},
  pages={431-9}
}
Conditional gene targeting and transgenic strategies utilizing Cre recombinase have been successfully applied to the analysis of development in mouse embryos. To create a conditional system applicable to heart progenitor cells, a Cre recombinase gene linked at its 5' end to an internal ribosome entry site (IRES) was inserted into the 3' untranslated region of the cardiac homeobox gene Nkx2-5 using gene targeting. Nkx2-5IRESCre mice were fully viable as homozygotes. We evaluated the efficacy of… CONTINUE READING

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