Efficient Co-Transformation of Nicotiana Tabacum by two Independent T-DNAs, the Effect of T-DNA Size and Implications for Genetic Separation

@article{McCormac2001EfficientCO,
  title={Efficient Co-Transformation of Nicotiana Tabacum by two Independent T-DNAs, the Effect of T-DNA Size and Implications for Genetic Separation},
  author={Alex C. McCormac and M. R. Fowler and Dong-Fang Chen and Malcolm C. Elliott},
  journal={Transgenic Research},
  year={2001},
  volume={10},
  pages={143-155}
}
The co-transformation of a single plant genome with two independent T-DNA regions provides opportunities for genetic separation in subsequent generations. In an effective strategy, co-delivery events must form a high proportion of the total transformed population. In this study, using the model plant species tobacco (Nicotiana tabacum), it was shown that the frequency of co-transformation within a given T0 population could be as high as 100% and this was found to be dependent, at least in part… CONTINUE READING

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