Efficiency and Impact of Positive and Negative Magnetic Separation on Monocyte Derived Dendritic Cell Generation.

Abstract

BACKGROUND The immunomagnetic separation technique is the basis of monocyte isolation and further generation of monocyte-derived dendritic cells. OBJECTIVE To compare the efficiency of monocyte positive and negative separation, concentration of beads, and their impact on generated dendritic cells. METHODS Monocytes were obtained using monoclonal… (More)

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