Efficacy of ImageJ in the assessment of apoptosis

  title={Efficacy of ImageJ in the assessment of apoptosis},
  author={Iman M Helmy and Adel M Abdel Azim},
  booktitle={Diagnostic pathology},
OBJECTIVE To verify the efficacy of ImageJ 1.43 n in determining the extent of apoptosis which is a complex and multistep process. STUDY DESIGN Cisplatin in different concentrations was used to induce apoptosis in cultured Hep2 cells. Cell viability assay and nuclear image analysis of stained Hep2 cells were used to discriminate apoptotic cells and cells suspected to be undergoing apoptosis from control cells based on parameters such as nuclear area, circularity, perimeter and nuclear area… CONTINUE READING