Effects on sialic acid recognition of amino acid mutations in the carbohydrate-binding cleft of the rotavirus spike protein.

  title={Effects on sialic acid recognition of amino acid mutations in the carbohydrate-binding cleft of the rotavirus spike protein.},
  author={Mark J Kraschnefski and Andrea Bugarcic and F. Fleming and Xing Yu and M. von Itzstein and B. Coulson and H. Blanchard},
  volume={19 3},
  • Mark J Kraschnefski, Andrea Bugarcic, +4 authors H. Blanchard
  • Published 2009
  • Biology, Medicine
  • Glycobiology
  • The rotavirus spike protein VP4 mediates attachment to host cells and subsequent membrane penetration. The VP8(*) domain of VP4 forms the spike tips and is proposed to recognize host-cell surface glycans. For sialidase-sensitive rotaviruses such as rhesus (RRV), this recognition involves terminal sialic acids. We show here that the RRV VP8(*)(64-224) protein competes with RRV infection of host cells, demonstrating its relevance to infection. In addition, we observe that the amino acids revealed… CONTINUE READING
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