Effects on growth and sporulation of inactivation of a Bacillus subtilis gene (ctc) transcribed in vitro by minor vegetative cell RNA polymerases (E-σ37, E-σ32)

@article{Truitt2004EffectsOG,
  title={Effects on growth and sporulation of inactivation of a Bacillus subtilis gene (ctc) transcribed in vitro by minor vegetative cell RNA polymerases (E-σ37, E-σ32)},
  author={Christine L. Truitt and E. Axelrad Weaver and William G. Haldenwang},
  journal={Molecular and General Genetics MGG},
  year={2004},
  volume={212},
  pages={166-171}
}
The E-σ37 gene ctc was inactivated by a site-specific insertion into the Bacillus subtilis chromosome. The resulting mutation inhibited sporulation by 95% at elevated temperatures (48° C). If the ctc - mutation is placed in a strain that carries a mutation in the closely linked but distinct spoVC gene, ctc now affects both growth and sporulation at elevated temperatures. Growth of the ctc - spoVC285 strain was transiently inhibited when exponentially growing cultures were shifted from 37° C to… CONTINUE READING

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