Effects of water pH and proteinase K treatment on the yield of environmental DNA from water samples

  title={Effects of water pH and proteinase K treatment on the yield of environmental DNA from water samples},
  author={Satsuki Tsuji and Hiroki Yamanaka and Toshifumi Minamoto},
Environmental DNA (eDNA) analysis has recently been applied to the study of aquatic macroorganisms. In most studies, sample water was filtered and the extracted DNA from the residues on the filter used for the following molecular analysis to detect species of interest. This quick, new biomonitoring method has received broad attention, but some unknowns remain, such as the eDNA yield in relation to water quality. Previous studies suggest that eDNA is composed of various forms, such as the free… 

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