Effects of vitrification medium composition on the survival of bovine in vitro produced embryos, following in straw-dilution, in vitro and in vivo following transfer.

@article{Pugh2000EffectsOV,
  title={Effects of vitrification medium composition on the survival of bovine in vitro produced embryos, following in straw-dilution, in vitro and in vivo following transfer.},
  author={Philip J. A. Pugh and H Robin Tervit and Heiner Niemann},
  journal={Animal reproduction science},
  year={2000},
  volume={58 1-2},
  pages={9-22}
}
This study examined the effects of adding a macromolecule, polyvinylpyrrolidone (10% PVP) and a sugar (0.3 M trehalose) to vitrification solutions (VS) containing either one (40% ethylene glycol [EG], two (25% EG+25% DMSO) or three (20% EG+20% DMSO+10% 1, 3-butanediol [BD]) permeable cryoprotectants on the survival and hatching of IVP bovine embryos, following vitrification, warming and in-straw cryoprotectant dilution. Grade 1 and 2 compact morulae and blastocysts were selected on Day 7 (Day 0… CONTINUE READING

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  • Aboul-Ela, M B And Barkawi, +21 authors C A And Molero-Filho

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