Effects of various cryoprotective agents and membrane-stabilizing compounds on bull sperm membrane integrity after cooling and freezing.

@article{Leeuw1993EffectsOV,
  title={Effects of various cryoprotective agents and membrane-stabilizing compounds on bull sperm membrane integrity after cooling and freezing.},
  author={F-E. de Leeuw and A M de Leeuw and J H den Daas and Ben Colenbrander and Arie J. Verkleij},
  journal={Cryobiology},
  year={1993},
  volume={30 1},
  pages={32-44}
}
In this study attempts were made to improve the survival rates of bull spermatozoa after freezing/thawing and to clarify the importance of certain agents to the cryopreservation of spermatozoa. For that purpose the standard freezing extender was modified by the addition of different concentrations of various cryoprotectants and membrane-stabilizing agents: glycerol, 1,2-propanediol, polyvinylpyrrolidone, sucrose, egg yolk, lipid vesicles, and bovine serum albumin (BSA). Sperm membrane… CONTINUE READING