Effects of vacuum UV and UVC radiation on dry E. coli plasmid pUC19. I. Inactivation, lacZ- mutation induction and strand breaks.

@article{Wehner1995EffectsOV,
  title={Effects of vacuum UV and UVC radiation on dry E. coli plasmid pUC19. I. Inactivation, lacZ- mutation induction and strand breaks.},
  author={J{\"u}rgen Wehner and Gerda Horneck},
  journal={Journal of photochemistry and photobiology. B, Biology},
  year={1995},
  volume={28 1},
  pages={
          77-85
        }
}
Using Escherichia coli plasmid pUC19 as a test system to study the effects of radiation on DNA at the molecular level, the wavelength (160-254 nm) dependence of inactivation (loss of the ability to transform E. coli), mutation induction in the target gene lacZ and induction of single-strand breaks and double-strand breaks was investigated. The same fluences were applied for all endpoints tested. In the UVC range, the cross-sections of inactivation and mutation induction match the DNA absorption… CONTINUE READING
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