Effects of urea induced protein conformational changes on ion exchange chromatographic behavior.

@article{Hou2010EffectsOU,
  title={Effects of urea induced protein conformational changes on ion exchange chromatographic behavior.},
  author={Ying Yu Hou and Thomas Budde Hansen and Arne Staby and Steven M. Cramer},
  journal={Journal of chromatography. A},
  year={2010},
  volume={1217 47},
  pages={7393-400}
}
Urea is widely employed to facilitate protein separations in ion exchange chromatography at various scales. In this work, five model proteins were used to examine the chromatographic effects of protein conformational changes induced by urea in ion exchange chromatography. Linear gradient experiments were carried out at various urea concentrations and the protein secondary and tertiary structures were evaluated by far UV CD and fluorescence measurements, respectively. The results indicated that… CONTINUE READING