Effects of ubiquitin system alterations on the formation and loss of a yeast prion.

@article{Allen2007EffectsOU,
  title={Effects of ubiquitin system alterations on the formation and loss of a yeast prion.},
  author={Kim D. Allen and Tatiana A Chernova and E Paula Tennant and Keith D. Wilkinson and Yury O Chernoff},
  journal={The Journal of biological chemistry},
  year={2007},
  volume={282 5},
  pages={3004-13}
}
The yeast prion [PSI+] is a self-propagating amyloidogenic isoform of the translation termination factor Sup35. Overproduction of the chaperone protein Hsp104 results in loss of [PSI+]. Here we demonstrate that this effect is decreased by deletion of either the gene coding for one of the major yeast ubiquitin-conjugating enzymes, Ubc4, or the gene coding for the ubiquitin-recycling enzyme, Ubp6. The effect of ubc4Delta on [PSI+] loss was increased by depletion of the Hsp70 chaperone Ssb but was… CONTINUE READING