Effects of transforming growth factor-beta1 on human vocal fold fibroblasts.

  title={Effects of transforming growth factor-beta1 on human vocal fold fibroblasts.},
  author={Ryan C. Branski and Silvia Stella Barbieri and Babette Weksler and Benjamin E Saltman and Priya Krishna and Dennis H. Kraus and Nalini V. Broadbelt and Jie Chen and Dix P. Poppas and Diane Felsen},
  journal={The Annals of otology, rhinology, and laryngology},
  volume={118 3},
OBJECTIVES We studied the effect of transforming growth factor (TGF)-beta on immortalized human vocal fold fibroblasts. METHODS Normal human vocal fold fibroblasts were subjected to sequential lentiviral transduction with genes for human telomerase (hTERT) and SV40 large T antigen in order to produce an "immortalized" cell line of normal phenotype. After confirmation of vocal fold fibroblast transfection, these cells, referred to as HVOX, were treated with various concentrations of exogenous… CONTINUE READING