Effects of trace elements in polymerization of keratin proteins and destruction of the filaments by processing techniques for electron microscopy.


Keratin extracted from keratinized cells of newborn rats polymerizes into filaments in vitro and this polymerization has been shown to be greatly stimulated by addition of divalent cations. In this study we investigated the optimum conditions for filament formation by use of CaCl2, ZnCl2, and GdCl3, a trivalent lanthanide. It was found that a concentration… (More)


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