Effects of three-dimensional culturing in a fibrous matrix on cell cycle, apoptosis, and MAb production by hybridoma cells.

@article{Luo2004EffectsOT,
  title={Effects of three-dimensional culturing in a fibrous matrix on cell cycle, apoptosis, and MAb production by hybridoma cells.},
  author={Jun Luo and Shangtian Yang},
  journal={Biotechnology progress},
  year={2004},
  volume={20 1},
  pages={306-15}
}
The effects of culturing hybridoma cells in a three-dimensional (3-D) poly(ethylene terephthalate) (PET) fibrous matrix on cell cycle, apoptosis, metabolism, and monoclonal antibody (MAb) production were evaluated by comparing with two-dimensional (2-D) culturing on microcarrier and multiwell plate surfaces. The percentage of cells in the G1/G0 phase increased during the long-term culturing period of approximately 4 weeks. Compared to the 2-D culture systems, cells grown in 3-D matrices had… CONTINUE READING

From This Paper

Topics from this paper.

Similar Papers

Loading similar papers…