Effects of the loss of capacity for N-glycosylation on the transport activity and cellular localization of the human reduced folate carrier.

@article{Wong1998EffectsOT,
  title={Effects of the loss of capacity for N-glycosylation on the transport activity and cellular localization of the human reduced folate carrier.},
  author={So C Wong and Liping Zhang and S A Proefke and Larry H. Matherly},
  journal={Biochimica et biophysica acta},
  year={1998},
  volume={1375 1-2},
  pages={6-12}
}
The role of N-glycosylation in reduced folate carrier (RFC) transport and membrane targeting was examined in transport-deficient K562 (K500E) cells transfected with human RFC cDNAs. Treatment of cells expressing wild-type RFC with tunicamycin (0-3 microg) resulted in a progressive shift of the approximately 85 kDa RFC on western blots to 65 kDa. At 3 microg/ml tunicamycin, the nearly complete loss of glycosylated RFC was accompanied by a approximately 25% decreased rate of methotrexate uptake… CONTINUE READING
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