Effects of the fluorescence dye DAPI on microtubule structure in vitro: formation of novel types of tubulin assembly products.

@article{Vater1993EffectsOT,
  title={Effects of the fluorescence dye DAPI on microtubule structure in vitro: formation of novel types of tubulin assembly products.},
  author={Wolfram Vater and Konrad Joachim B{\"o}hm and Eugen Unger},
  journal={Acta histochemica},
  year={1993},
  volume={94 1},
  pages={
          54-66
        }
}
It has been found that the DNA fluorescence dye 4',6-diamidino-2-phenylindole (DAPI) is able to stain also microtubules. However, electron microscopy revealed that DAPI changed microtubule structure and induced the formation of a broad spectrum of polymorphic tubulin assembly products. Upon addition of DAPI to microtubules assembled from 10 to 15 mumol tubulin (molar DAPI/tubulin ratios of 10 to 40) in the presence of microtubule-associated proteins, most of the microtubules were decorated with… CONTINUE READING

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