Effects of thawing, refreezing and storage conditions of tissue samples and protein extracts on 2-DE spot intensity.


We report that reliable quantitative proteome analyses can be performed with tissue samples stored at -80 degrees C for up to 10 years. However, storing protein extracts at 4 degrees C for 24 h and freezing protein extracts at -80 degrees C and thawing them significantly altered 41.6 and 17.5% of all spot intensities on 2-DE gels, respectively. Fortunately… (More)
DOI: 10.1002/pmic.200900471


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