Effects of synthetic gonadotropin-releasing hormone on incorporation of radioactive glucosamine and amino acids into luteinizing hormone and total protein by rat pituitaries in vitro.

  title={Effects of synthetic gonadotropin-releasing hormone on incorporation of radioactive glucosamine and amino acids into luteinizing hormone and total protein by rat pituitaries in vitro.},
  author={T C Liu and Gary L. Jackson and Jack Gorski},
  volume={98 1},
The influence of synthetic gonadotropin-releasing hormone (GnRH) on incorporation of glucosamine and amino acids into LH and total protein was investigated. Rat pituitaries were incubated in vitro with radioactive precursors for 4 h at 37 C. Labeled LH was isolated by immunoprecipitation with specific anti-LH-beta serum. Total LH in the medium and pituitary glands was measured by radioimmunoassay. GnRH significantly stimulated incorporation of [3H]glucosamine into LH in pituitaries from… 

Comparison of the biosynthesis and release of luteinizing hormone by rat pituitaries in vitro in response to gonadotropin-releasing hormone analogs.

In an attempt to determine whether gonadotropin-releasing hormone (GnRH) regulates synthesis and release of LH via independent processes, the ability of several GnRH analogs to regulate LH release

The effects of gonadotropin-releasing hormone and estradiol on luteinizing hormone biosynthesis in cultured rat anterior pituitary cells.

GnRH regulates both LH glycosylation and LH polypeptide synthesis and that E2 lowers the physiological concentration of GnRH necessary to stimulate this biosynthetic response, and estrogen's enhancement of Gn RH-stimulated LH release appears to be due to its action on mechanisms regulating the release of previously synthesized stored hormone.

Modifications of luteinizing hormone biosynthesis and release by gonadotropin-releasing hormone, cycloheximide, and actinomycin D.

The data suggest that the time course for the release of preexisting IR-LH differs from that for newly synthesised LH, and the newly synthesized LH released in response to high levels of GnRH has more sugar residues than that released under basal conditions.

Synthesis and release of luteinizing hormone in vitro by rat anterior pituitary cells: effects of gallopamil hydrochloride (D600) and pimozide.

The results demonstrate that D600 and pimozide inhibit both GnRH-induced LH glycosylation and release, which are both mediated by similar Ca2+-dependent pathways.

Characterization of luteinizing hormone synthesized and released by rat pituitaries in vitro: dissociation of immunological and biological activities.

The LH synthesized de novo by rat anterior pituitary glands in vitro by monitoring the radioactivity of LH immunoprecipitated with anti-LH-β serum was characterized and newly synthesized radiolabeled LH was measured.

Gonadotropin-releasing hormone increases the amount of messenger ribonucleic acid for gonadotropins in ovariectomized ewes after hypothalamic-pituitary disconnection.

It was considered that the pituitary gland remained viable and functioned independently of hypothalamic input in OVX ewes after HPD and there were no differences in the amount of mRNA for GH or PRL or the pituitsary content of these hormones among treatments.

Biosynthesis, Glycosylation, and Secretion of Rat Luteinizing Hormone α- and β-Subunits: Differential Effects of Orchiectomy and Gonadotropin-Releasing Hormone*

Neither orchiectomy nor GnRH had any effect on [35S]Cys or [3H]GlcN incorporation into intracellular or secreted total protei...